GABA-A receptors γ

Polyclonal guinea pig antibody against GABA-A receptor γ2
Cat. No. 224 004 crude antiserum	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: limited (see remarks) IP: yes ICC: 1 : 500 (see remarks) image IHC: 1 : 500 up to 1 : 2000 (see remarks) image
Immunogen	Synthetic peptide (aa 39 - 67 of mouse GABA-A receptor γ2 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for GABA-A receptor γ2.
Remarks	WB: The antibody is less sensitive in westernblotting compared to the rabbit antibody. This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. IHC: For best results use the protocol of Schneider Gasser et al., 2006.