GABA-A receptors α

Receptors mediating inhibitory neurotransmission

Fact Sheet

Polyclonal rabbit antibody against GABA-A receptor α2
Cat. No. 224 102 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 224 103 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining; see remarks) blot IP: not tested yet ICC: 1 : 500 (see remarks) image IHC: 1 : 1000 (see remarks) image
Immunogen	Synthetic peptide (aa 29 - 37 of rat GABA-A receptor α2 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for GABA-A receptor α2.
Remarks	WB: The crude antiserum is highly recommended. This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC, IHC: The affinity purified antibody is highly recommended for these applications. For best results use the protocol of Schneider Gasser et al., 2006. ICC: This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.

Polyclonal guinea pig antibody against GABA-A receptor α2
Cat. No. 224 104 crude antiserum	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining; see remarks) blot IP: not tested yet ICC: 1 : 200 up to 1 : 500 (see remarks) image IHC: not tested yet
Immunogen	Synthetic peptide (aa 29 - 37 of rat GABA-A receptor α2 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for GABA-A receptor α2.
Remarks	WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.

Control peptide for GABA-A receptor α2 antibodies
Cat. No. 224-1P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (aa 29 - 37 of rat GABA-A receptor α2 precursor protein) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Polyclonal rabbit antibody against GABA-A receptor α1
Cat. No. 224 203 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 500 (see remarks) image IHC: 1 : 1000 up to 1 : 5000 (see remarks) image
Immunogen	Synthetic peptide (aa 28 - 43 of rat GABA-A receptor α1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for GABA-A receptor α1.
Remarks	WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. IHC: For best results use the protocol of Schneider Gasser et al., 2006.