γ-Enolase
A neuron-specific enolase subunit
Fact Sheet Cat. No. 230 002, 230 003
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 230 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 230 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 10000 (AP staining) 230 002 230 003
IP: yes ICC: not tested yet IHC: 1 : 1000 up to 1 : 5000 (see remarks) 230 003 |
| Immunogen | Synthetic peptide (aa 418 - 434 of rat γ-enolase) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for γ-enolase. |
| Remarks | IHC: The affinity purified antibody only. The crude antiserum is not recommended. |
