Endophilin (SH3P4, SH3GL2)
An SH3 domain protein involved in clathrin mediated endocytosis
Fact Sheet
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 159 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 10 000 (AP staining) blot
IP: not tested yet ICC: 1 : 500 image IHC: 1 : 1000 image |
| Immunogen | Synthetic peptide QPKPRMSLEFATGDSTQ (aa 256 - 276 in mouse endophilin 1) coupled to KLH via an N-terminal cysteine. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for endophilin 1. |
| Control peptide | |
| Cat. No. 159-0P |
100 µg lyophilized peptide. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized! |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control peptide consists of the synthetic peptide (QPKPRMSLEFATGDSTQ) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
