ELKS (ERCs/CASTs)

RIM binding proteins

Fact Sheet

Polyclonal rabbit antibody against ERC 1b/2
Cat. No. 143 003 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in TBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 100 up to 1 : 5000 (AP staining) blot IP: not tested yet ICC: 1 : 1000 image IHC: 1 : 100 image
Immunogen	Synthetic peptide CDQDEEEGIWA (aa 939 - 948 in rat ERC 1b and aa 912 - 921 in ERC 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, hamster; other species not tested yet.
Specificity	Specific for the brain specific isoforms ERC 1b and ERC 2.

Control protein
Cat. No. 143-0P	100 µg lyophilized protein. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use.
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control protein consists of the C-terminus of rat ERC 1b (aa 578 - 948). It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Polyclonal rabbit antibody against ERC 2
Cat. No. 143 103 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in TBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 100 up to 1 : 2000 (AP staining) blot IP: not tested yet ICC: not recommended IHC: not recommended
Immunogen	Synthetic peptide CDIEDDSRMNPEFADRLK (aa 655 - 670 in rat ERC 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Rat, mouse, hamster; other species not tested yet.
Specificity	Specific for ERC 2.

Control peptide
Cat. No. 143-1P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (CDIEDDSRMNPEFADRLK) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.