Dynamin

GTPase involved in endocytosis

Fact Sheet

Polyclonal rabbit antibody against dynamin 1, 2, 3
Cat. No. 115 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 200 up to 1 : 1000 image IHC: 1 : 100 image
Immunogen	Synthetic peptide GNRGMEDLIPLVNRLQ (aa 2 - 17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, cow.
Specificity	Recognizes dynamin 1, 2 and 3.

Control peptide for dynamin 1, 2, 3
Cat. No. 115-0P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (GNRGMEDLIPLVNRLQ) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Polyclonal rabbit antibody against dynamin 3
Cat. No. 115 302 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) blot IP: yes ICC: 1 : 500 up to 1 : 1000 image IHC: not tested yet
Immunogen	Synthetic peptide (aa 773 - 794 in mouse dynamin 3) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for dynamin 3.

Control peptide for dynamin 3
Cat. No. 115-3P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (aa 773 - 794 in mouse dynamin 3) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.