| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 189 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 189 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining; see remarks) 189 002
IP: not tested yet ICC: 1 : 500 (see remarks!) 189 003 IHC: not tested yet |
| Immunogen |
189 002: Mixture of synthetic peptides of rat densin variant A (aa 877 - 891 and aa 1341 - 1357) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. 189 003: Synthetic peptide of rat densin (aa 877 - 891) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specidic for densin 180. |
| Remarks | The crude antiserum is recommended for WB (stronger signals). It is not recommended for ICC. |