| Polyclonal guinea pig antibody | |
|---|---|
|
Cat. No. 239 005 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Guinea pig serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: 1 : 200 up to 1 : 500 (see remarks) IHC: not tested yet |
| Immunogen | Recombinant protein of human CLIP 170 (aa 1283 - 1438). |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Recognizes CLIP 170 and restin. |
| Remarks | ICC: Fix 15 min at -20°C with ice-cold MeOH / 1mM EGTA, then transfer without washing to 4% PFA (in PBS) and fix for 20 min at RT. |