| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 241 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining; see remarks) blot
IP: not tested yet ICC: 1 : 500 image IHC: 1 : 500 image |
| Immunogen | Synthetic peptide (aa 188 - 207 in rat claudin 11) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. |
| Reactivity | Human, rat, mouse, pig, cow. |
| Specificity | Specific for claudin 11. |
| Remarks | WB: Moderate heating (50°C) of samples prior SDS-PAGE improves signal strength. |