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Cellubrevin (VAMP 3)

Major vesicle protein involved in fusion

Fact Sheet Cat. No. 104 102, 104 203

Polyclonal rabbit antibody against VAMP 1/2/3
Cat. No. 104 102
crude antiserum
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Cat. No. 104 203
purified antibody
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) 104 102    104 203
IP: yes (cat. No. 104 102 tested only)
ICC: 1 : 500 (see remarks) ) 104 102    104 203
IHC: not tested yet
ELISA: yes
Immunogen Recombinant protein of the cytoplasmic part of rat cellubrevin (aa 1 - 81).
Reactivity Human, rat, mouse; other species not tested yet.
Specificity Specific for all three isoforms VAMP 1, 2 and 3.
Remarks
  • ICC: Methanol and acetone fixation; see Annaert et al., 1997
  • This antibody is recommended for ICC of non-neuronal cells.
  • This antibody detects the tetanus and Botulinum B toxin cleavage product in toxin treated tissue homogenates.





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Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com | Imprint
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com