Caveolin 1
The major coat protein of caveolae
Fact Sheet
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 161 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 161 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) 161 003
IP: yes ICC: 1 : 100 up to 1 : 500 161 003 IHC: 161 002: not tested yet 161 003: yes image |
| Immunogen | Synthetic peptide MSGGKYVDSEGHLYTVP (aa 1 - 17) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue. |
| Reactivity | Human, rat, mouse, cow, dog, pig, monkey. |
| Specificity | Specific for cavaeolin 1. |
| Control peptide | |
| Cat. No. 161-0P |
100 µg lyophilized peptide. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized! |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control peptide consists of the synthetic peptide (MSGGKYVDSEGHLYTVP) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
