Calbindins

Vitamin D-dependent calcium binding proteins

Fact Sheet

Monoclonal mouse antibody against calbindin D28k
calbindin D28k calbindin D29k
Cat. No. 214 011 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: not tested yet IHC: 1 : 200 image
Clone	351C10
Subtype	IgG1
Immunogen	Recombinant protein of human calbindin D28k (aa 3 - 251).
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for calbindin D28k.

Polyclonal rabbit antibody against calbindin D28k
Cat. No. 214 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) blot IP: not tested yet ICC: not tested yet IHC: 1 : 500 image
Immunogen	Recombinant protein of human calbindin D28k (aa 3 - 251).
Reactivity	Human, rat, mouse, monkey, ape, cow.
Specificity	Specific for calbindin D28k.

Polyclonal guinea pig antibody against calbindin D28k
Cat. No. 214 004 crude antiserum	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) blot IP: not tested yet ICC: not tested yet IHC: 1 : 200 up to 1 : 500 image
Immunogen	Recombinant protein of human calbindin D28k (aa 3 - 251).
Reactivity	Human, rat, mouse, monkey, ape, cow.
Specificity	Specific for calbindin D28k.

Polyclonal rabbit antibody against calbindin D29k / calretinin
Cat. No. 214 102 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) blot IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) image
Immunogen	Recombinant full length mouse calbindin D29k.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for calbindin D29k.
Remarks	Works also well on paraffin embedded material.

Polyclonal guinea pig antibody against calbindin D29k / calretinin
Cat. No. 214 104 crude antiserum	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: not tested yet IHC: 1 : 500 image
Immunogen	Recombinant full length mouse calbindin D29k.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for calbindin D29k.

Control protein for calbindin D29k / calretinin antibodies
Cat. No. 214-1P	100 µg lyophilized protein. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use.
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control protein consists of the recombinant protein (full length mouse calbindin D29k) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

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