Bassoon

A large multi-domain protein of the presynaptic active zone

Fact Sheet

Monoclonal mouse antibody
Cat. No. 141 021 hybridoma supernatant	200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 100 up to 1 : 500 (see remarks) image IHC: not tested yet ELISA: yes (see remarks)
Clone	219E1
Subtype	IgG2b
Immunogen	Recombinant protein containing the 330 C-terminal aa of rat bassoon.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for bassoon.
Remarks	ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 141 003 as detector antibody (protocol for sandwich-ELISA).

Polyclonal rabbit antibody
Cat. No. 141 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 141 003 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining) 141 002 IP: not tested yet ICC: 1 : 200 up to 1 : 2000 141 002 141 003 IHC: yes ELISA: yes (cat. no. 141 003; see remarks)
Immunogen	Recombinant protein containing the 330 C-terminal aa of rat bassoon.
Reactivity	Rat, mouse, no signal in chicken; other species not tested yet.
Specificity	Specific for bassoon.
Remarks	Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed. ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 141 021 as capture antibody (protocol for sandwich-ELISA).

Polyclonal guinea pig antibody
Cat. No. 141 004 crude antiserum	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 500 image IHC: not tested yet
Immunogen	Recombinant protein containing the 330 C-terminal aa of rat bassoon.
Reactivity	Rat, mouse, no signal in chicken; other species not tested yet.
Specificity	Specific for bassoon.
Remarks	Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed.

Control protein for all antibodies
Cat. No. 141-0P	100 µg lyophilized protein. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use.
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control protein consists of the recombinant protein that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.