Bassoon
A large multi-domain protein of the presynaptic active zone
Fact Sheet Cat. No. 141 021
| Monoclonal mouse antibody | |
|---|---|
|
Cat. No. 141 021 hybridoma supernatant |
200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: 1 : 100 up to 1 : 500 (see remarks) image IHC: not tested yet ELISA: yes (see remarks) |
| Clone | 219E1 |
| Subtype | IgG2b |
| Immunogen | Recombinant protein containing the 330 C-terminal aa of rat bassoon. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for bassoon. |
| Remarks |
ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 141 003 as detector antibody (protocol for sandwich-ELISA). |
