Arc

A cytoskeleton-associated protein involved in neuronal plasticity

Fact Sheet

Monoclonal mouse antibody
Cat. No. 156 011 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: not recommended IP: yes ICC: not tested yet IHC: not tested yet ELISA: yes (see remarks)
Clone	48B2
Subtype	IgG2b
Immunogen	Strep-Tag® fusion protein of full-length mouse arc.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for Arc.
Remarks	ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 156 003 as detector antibody (protocol for sandwich-ELISA).

Polyclonal rabbit antibody
Cat. No. 156 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 156 003 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 500 up to 1 : 2000 (AP staining) blot IP: yes ICC: 1 : 1000 (see remarks) IHC: 1 : 1000 (see remarks) 156 003 ELISA: yes (cat. No. 156 003; see remarks)
Immunogen	Strep-Tag® fusion protein of full-length mouse arc.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for arc.
Remarks	ICC/IHC: The affinity purified antibody is recommended. This antibody works also well on paraffin embedded material. ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 156 011 as capture antibody (protocol for sandwich-ELISA).

Polyclonal guinea pig antibody
Cat. No. 156 005 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Guinea pig serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 2000 (AP staining) blot IP: not tested yet ICC: 1 : 1000 IHC: 1 : 1000 image
Immunogen	Strep-Tag® fusion protein of full-length mouse arc.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for arc.

Control protein
Cat. No. 156-0P	100 µg lyophilized protein. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use.
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control protein consists of the full length recombinant protein that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.