GluA (AMPA, GluR)
A member of a main group for rapid excitatory neurotransmission in the mammalian CNS
Fact Sheet Cat. No. 182 403
| Polyclonal rabbit antibody against GluA 1-4 (AMPA 1-4) | |
|---|---|
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Cat. No. 182 403 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: not tested yet IHC: 1 : 100 up to 1 : 500 (see remarks) image |
| Immunogen | Recombinant protein of the extracellular epitope of rat GluA 1 (aa 742 - 798). |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Raised against GluA 1 but, due to sequence homology, likely to crossreact with GluA 2, 3, and 4. |
| Remarks |
IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
The antibody is also suitable for EM based replica technique. |
