GluA 1 (AMPA 1, GluR 1)

A member of a main group for rapid excitatory neurotransmission in the mammalian CNS

Fact Sheet

Monoclonal mouse antibody
Cat. No. 182 011 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 200 image IHC: yes image
Clone	160E5
Subtype	IgG2b
Immunogen	Synthetic peptide (aa 895 - 907 in rat GluA 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for GluA 1.

Control peptide for mouse antibody
Cat. No. 182-01P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (aa 895 - 907 in rat GluA 1) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Polyclonal rabbit antibody
Cat. No. 182 003 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 500 (see remarks) image IHC: not tested yet
Immunogen	Recombinant protein of mouse GluA 1 (C-terminus; aa 828 - 906).
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for GluA 1.
Remarks	ICC: Ice cold methanol fixation recommended.

Control protein for rabbit antibody
Cat. No. 182-0P	100 µg lyophilized protein. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use.
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control protein consists of the recombinant protein (aa 828 - 906 of mouse GluA 1) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.