α/β/γ/ SNAP

Peripheral membrane proteins required for membrane traffic

Fact Sheet

Monoclonal mouse antibody, clone 77.1
α/β SNAP β SNAP γ SNAP
Cat. No. 112 011 IgG, unlabeled	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) blot IP: yes (see remarks) ICC: no (denaturation of the antigen during fixation) IHC: not tested yet
Clone	77.1
Subtype	IgG2b
Immunogen	Recombinant full length rat α SNAP
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for α- and β SNAP, does not cross-react to γ SNAP.
Remarks	The antibody does not immunoprecipitate the 20 S SNARE-complex.

Monoclonal mouse antibody, clone 77.2
Cat. No. 112 111 IgG, unlabeled	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) blot IP: yes (see remarks) ICC: 1 : 500 up to 1 : 1000 image IHC: not tested yet
Clone	77.2
Subtype	IgG1
Immunogen	Recombinant full length rat α SNAP.
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for α- and β SNAP, does not cross-react to γ SNAP.
Remarks	The antibody does not immunoprecipitate the 20 S SNARE-complex.

Polyclonal rabbit antibody against β SNAP
Cat. No. 112 203 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 200 up to 1 : 1000 (AP staining) blot IP: yes ICC: not tested yet IHC: not tested yet
Immunogen	Synthetic peptide IKKSIQGDGEGDGDLK (aa 283 - 298 in mouse β SNAP) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, cow.
Specificity	Specific for β SNAP.

Control peptide for β SNAP antibody
Cat. No. 112-2P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (IKKSIQGDGEGDGDLK) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Polyclonal rabbit antibody against γ SNAP
Cat. No. 112 303 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 500 up to 1 : 2000 (AP staining) blot IP: yes ICC: not tested yet IHC: not tested yet
Immunogen	Synthetic peptide AAQKINEGLEHLAKAEK (2 - 18 in rat γ SNAP) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, cow.
Specificity	Specific for γ SNAP.

Control peptide for γ SNAP antibody
Cat. No. 112-3P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (AAQKINEGLEHLAKAEK) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.