| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 167 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 5000 (AP staining) blot
IP: not tested yet ICC: not tested yet IHC: not tested yet |
| Immunogen | Recombinant full length human α internexin. |
| Reactivity | Rat; other species not tested yet. |
| Specificity | Specific for α internexin. |
| Control protein | |
| Cat. No. 167-0P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant protein that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |