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MLC-2A protein - 311-0P

Myosin light chain 2A or MLC-2A is specific for the atrium of the mammalian heart
control protein
Cat. No.: 311-0P
Amount: 100 µg
Price: $105.00
Cat. No. 311-0P 100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.
Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
Immunogen Recombinant protein corresponding to AA 1 to 175 from human MLC-2A (UniProt Id: Q01449)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 311 011, 311 011AT647N, 311 018
Remarks

This control protein consists of the recombinant protein that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 311-0p.pdf
Cat. No.: 311-0P
Amount: 100 µg
Price: $105.00
Background

During cardiogenesis two major isoforms of myosin light chain 2 are co-expressed in a tightly regulated manner. MLC-2A is only present in the atrium while MLC-2V is exclusively expressed in the ventricle. Knock out studies revealed that the 2A isoform cannot substitute for the 2V variant in the ventricular chamber.
Recently it has been demonstrated that embryonic and adult stem cells can be differentiated into cardiomyocytes which may generate suitable replacements for damaged heart tissue in the future.
This monoclonal antibody is a useful tool to distinguish between ventricle and atrium specific cardiomyocytes.

Protocols