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Home > Products > 135 011BT

VGLUT1 antibody - 135 011BT K.O.

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
Mouse monoclonal purified IgG
Cat. No.: 135 011BT
Amount: 100 µg
Price: $470.00
Cat. No. 135 011BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 up to 1 : 1000 gallery  
Label biotin
Clone 68B7
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: mouse (Q3TXX4), rat (Q62634), human (Q9P2U7).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 135-0P
Remarks

WB: This antibody is highly recommended for Western blot experiments. VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples
Data sheet 135_011bt.pdf

References for VGLUT1 - 135 011BT

Multiple genes in a single GWAS risk locus synergistically mediate aberrant synaptic development and function in human neurons.
Zhang S, Zhang H, Forrest MP, Zhou Y, Sun X, Bagchi VA, Kozlova A, Santos MD, Piguel NH, Dionisio LE, Sanders AR, et al.
Cell genomics (2023) 39: 100399. 135 011BT ICC; tested species: human
Cat. No.: 135 011BT
Amount: 100 µg
Price: $470.00
Multiple genes in a single GWAS risk locus synergistically mediate aberrant synaptic development and function in human neurons.
Zhang S, Zhang H, Forrest MP, Zhou Y, Sun X, Bagchi VA, Kozlova A, Santos MD, Piguel NH, Dionisio LE, Sanders AR, et al.
Cell genomics (2023) 39: 100399. 135 011BT ICC; tested species: human
WB
ICC
IHC
IHC
IHC-P
IHC-P
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.
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