Cat. No. 111 011BT |
50 µg purified IgG, lyophilized, labeled with Biotin.
Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes (see remarks) Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 100 up to 1 : 1000 Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not recommended Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet |
Label | biotin |
Clone | 71.1 |
Subtype | IgG1 (κ light chain) |
Immunogen | Recombinant protein corresponding to AA 1 to 206 from rat SNAP25B (UniProt Id: P60881-1) |
Epitop |
Epitop: AA 20 to 40 from rat SNAP25B (UniProt Id: P60881-1) |
Reactivity |
Reacts with: human (P60880), rat (P60881), mouse (P60879), vertebrates, invertebrates. Other species not tested yet. |
Specificity | Detects both splice variants SNAP 25A and B. Recognizes the Botulinum neurotoxin A and E cleavage products. |
Remarks |
IP: Immunoprecipitation not quantitative, appears to depend on the binding status of the protein. |
Data sheet | 111_011bt.pdf |
SNAP 25 (synaptosome-associated protein of 25 kDa) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP 25 is the target of Botulinum neurotoxin A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP 25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin 1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP 25A and 25B.